Protein Engineering of Antifreeze Proteins Reveals that Their Activity Scales with the Area of the Ice-Binding Site

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FEBS Letters - 2022 - Scholl - Protein engineering of antifreeze proteins reveals that their activity scales with the area.pdf (8.67 MB)

Date

2022-12-02

Authors

Scholl, Connor L.
Davies, Peter

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Wiley

Abstract

Antifreeze proteins (AFPs) protect organisms from freezing by binding to ice crystals to prevent their growth. Here, we have investigated how the area of an AFP's ice-binding site (IBS) changes its antifreeze activity. The polyproline type II helical bundle fold of the 9.6-kDa springtail (Collembola) AFP from Granisotoma rainieri (a primitive arthropod) facilitates changes to both IBS length and width. A one quarter decrease in area reduced activity to less than 10%. A one quarter increase in IBS width, through the addition of a single helix, tripled antifreeze activity. However, increasing IBS length by a similar amount actually reduced activity. Expanding the IBS area can greatly increase antifreeze activity but needs to be evaluated by experimentation on a case-by-case basis.

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This is the peer reviewed version of the following article: Scholl, C.L. and Davies, P.L. (2022), Protein engineering of antifreeze proteins reveals that their activity scales with the area of the ice-binding site. FEBS Lett. Accepted Author Manuscript. https://doi.org/10.1002/1873-3468.14552, which has been published in final form at https://doi.org/10.1002/1873-3468.14552. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions. This article may not be enhanced, enriched or otherwise transformed into a derivative work, without express permission from Wiley or by statutory rights under applicable legislation. Copyright notices must not be removed, obscured or modified. The article must be linked to Wiley’s version of record on Wiley Online Library and any embedding, framing or otherwise making available the article or pages thereof by third parties from platforms, services and websites other than Wiley Online Library must be prohibited.

Keywords

Antifreeze Protein, Protein Engineering, Polyproline Type II Helix, Collembola, Surface Waters, Ice-Binding

Citation

Scholl, C.L. and Davies, P.L. (2022), Protein engineering of antifreeze proteins reveals that their activity scales with the area of the ice-binding site. FEBS Lett. Accepted Author Manuscript. https://doi.org/10.1002/1873-3468.14552

URI

http://hdl.handle.net/1974/31336

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Department of Biomedical and Molecular Sciences Faculty Publications

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